Crystallization of mutated T1 Lipase from thermostableGeobacillus zalihaestrain T1

Improvement of Thermal Stability via Outer-Loop Ion Pair Interaction of Mutated T1 Lipase from Geobacillus zalihae Strain T1

Mutant D311E and K344R were constructed using site-directed mutagenesis to introduce an additional ion pair at the inter-loop and the intra-loop, respectively, to determine the effect of ion pairs on the stability of T1 lipase isolated from Geobacillus zalihae. A series of purification steps was applied, and the pure lipases of T1, D311E and K344R were obtained. The wild-type and mutant lipases...

A) Stream a T2/t1(stream A) T3/t1(local Stream) T2/t1(local Stream) T3/t1(stream A) (a) Stream a T2/t1(stream A) T3/t1(local Stream) T2/t1(local Stream) T3/t1(stream A

25 Modeling and simulation of a network interface handling two priority levels. We propose a scheme called Double Queue Scheme to reduce the eeects of local jobs' communication on the performance of parallel jobs. It divides the only one input and output queues in traditional network interfaces into two ones. One is for the communication of parallel jobs, and the other is for the communication ...

T1: Multimedia

Accurate Estimation of T1 from SPGR Signals

Introduction T1 maps can be computed from spoiled gradient recalled echo (SPGR) images acquired with different flip angles and/or repetition times (TRs). The function relating signal intensity to flip angle and TR is non-linear; however, a linear form proposed by Gupta in 1977 [1] is currently widely used [1-6]. Using this linearized model, T1 has been estimated with a linear least squares (LLS...

Brain segmentation performance using T1-weighted images versus T1 maps

The recent driven equilibrium single-pulse observation of T1 (DESPOT1) approach permits real-time clinical acquisition of large-volume and high-isotropic-resolution T1 mapping of MR tissue parameters with improved uniformity. It is assumed that the quantitative nature of maps will facilitate clinical applications such as disease diagnosis and comparison across subjects. However, there is not ye...